By the grace of Almighty God, and in the presence of Professor Dr. Bashar Sadiq Noumi, Dean of the College of Veterinary Medicine at the University of Tikrit, the Master’s thesis entitled:
“Evaluation of the Protective Efficacy of Inactivated Vaccines against Aeromonas hydrophila in Common Carp Fish”
submitted by the graduate student Reem Zaidan Mukhlif, in the field of Microbiology, was discussed at the College of Veterinary Medicine, University of Tikrit.
The examination committee consisted of:
Professor Dr. Jalal Yassin Mustafa
(Microbiology) – University of Basrah, College of Veterinary Medicine
Chairman
Assistant Professor Dr. Hassan Hadi Khurshid
(Animal Diseases) – University of Tikrit, College of Veterinary Medicine
Member
Assistant Professor Dr. Sana Saud Ahmed
(Microbiology) – University of Tikrit, College of Veterinary Medicine
Member
Assistant Professor Dr. Aghareed Ali Hussein
(Immunology) – University of Tikrit, College of Veterinary Medicine
Member and Supervisor
The thesis focused on evaluating the immune and protective responses of inactivated vaccines using two methods: injection and immersion (spray). The results showed that IgT levels were more stable with a noticeable hepatic effect, without significant differences in the overall disease progression. In addition, the results of lncRNA (MSTRG.111162.1) indicated no significant overall differences among the groups, with a clear decrease after challenge in the injection group compared to the immersion method.
The study also demonstrated that survival rates and immune responses were higher using the injection method compared to immersion, indicating a longer systemic stability with injection versus a faster but shorter mucosal response with immersion. The findings further confirmed that using local bacterial isolates in vaccine preparation provides better and faster protection, as they are more closely related to field strains. Accordingly, the study recommended adopting a prime–boost strategy, using immersion as a primer and injection as a booster.
The discussion, which was held in Dr. Muhannad Maher Hall at the College, was attended by a number of faculty members as well as postgraduate and undergraduate students.
At the conclusion of the discussion, the Dean of the College commended the scientific efforts exerted and wished the student continued success, reaffirming the College’s commitment to supporting rigorous scientific research that serves both the academic and productive sectors.
